Bacillus subtilis Early Colonization of Arabidopsis thaliana Roots Involves Multiple Chemotaxis Receptors

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Bacillus subtilis Early Colonization of Arabidopsis thaliana Roots Involves Multiple Chemotaxis Receptors

Colonization of plant roots by Bacillus subtilis is mutually beneficial to plants and bacteria. Plants can secrete up to 30% of their fixed carbon via root exudates, thereby feeding the bacteria, and in return the associated B. subtilis bacteria provide the plant with many growth-promoting traits. Formation of a biofilm on the root by matrix-producing B. subtilis is a well-established requireme...

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Colonization studies previously performed with a green-fluorescent-protein, GFP, labeled derivative of Bacillus amyloliquefaciens FZB42 revealed that the bacterium behaved different in colonizing surfaces of plant roots of different species (Fan et al., 2012). In order to extend these studies and to elucidate which genes are crucial for root colonization, we applied targeted mutant strains to A...

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The net methylation of methyl-accepting chemotaxis proteins (MCPs) is the biochemical manifestation of physiological adaptation to amino acid chemoattractants by Escherichia coli (4). In Bacillus subtilis, demethylation of MCPs occurs during such adaptation (3). The activity of chemotaxis methyltransferase is detectable in vivo and in vitro. As an alternative assay, whole cells can be permeabil...

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ژورنال

عنوان ژورنال: mBio

سال: 2016

ISSN: 2161-2129,2150-7511

DOI: 10.1128/mbio.01664-16